BioTech Bootcamp Summer 2010 Tentative

	Monday	Tuesday	Wednesday	Thursday	Friday	Monday
8:00 AM	Welcome (Classroom)	Recap of Monday & questions	Recap of Tuesday & questions	Recap of Wednesday & questions	Recap of Thursday & questions	Classroom Recap of last week
		Planning & Documenting Laboratory Dealing with complicated protocols	-Practical dilution exercise. -Plating bacteria to get a concentration.	Growing bacterial cells	Protein purification – GPF from pGLO transformed cells	Student Assessment of Biotech Bootcamp
9:00 AM	Registration (auditorium)					Associate Company roundtable discussion
				Centrifugation
			Measuring, adjusting and controlling pH		G-MOD lab II
10:00 AM	Tour of laboratory and floor (pix taken while on tour)	Using laboratory balances		Centrifugation exercise
			-Measuring pH of a liquid -Proper use of a pH meter -Calibrating a pH meter
		Reproducibility and Accuracy of Measurements – Data Analysis Using Excel		Bacterial plasmid miniprep	Protein assay – colorimetric	Research Lab roundatable discussions
11:00 AM	Introduction and general orientation
			Buffers – What are they and why are they used?	Spectrophotometry (cont.)
	General laboratory safety
12:00 PM	Lunch	Lunch	Lunch	Lunch	Lunch	Lunch
1:00 PM	Laboratory safety exercise	Non-sterile vs. sterile techniques	Buffers – comparison of titrating a salt solution and a buffer	Transformation	Enzyme catalysis	Interns report to their respective companies
		Preparation of LB broth and LB agar
	-Reading MSDS sheets -Finding safety information on chemical bottles
2:00 PM			Preparation of Glycine/Mg buffer (pH 7.0, pH 8.0, pH 9.0 & pH 10.0) Preparation of PNPP	G-MOD lab I
		Handling and measuring liquids II. Stock solutions and dilutions
3:00 PM	Units and the metric system
		-Preparing 1X TAE -Serial two-fold dilutions	Spectrophotometery	Lab Math IV -Representing data -Semi-log graph paper -Determining rates
	Handling & measuring liquids I. Pipets and other measuring devices				How to prepare Powerpoint material: A Powerpoint tutorial for posters and presentations
4:00 PM
		Reading a scientific paper	Reading a scientific paper (Part II)	Reading a scientific paper (Part III)
	Rainbow pipeting lab