Sequencing Projects

Large-scale genome sequencing is a core capability of HudsonAlpha. We offer two avenues to work with our sequencing teams.

High-Throughput Sequencing Service

The HudsonAlpha Genomic Services Lab offers cutting-edge genetic research tools to both academic and commercial customers. Take advantage of our service lab for sample processing. Cutting-edge genetic research tools to both academic and commercial customers.

  • No demands for authorship or data ownership
  • Automated processing to minimize variation and increase throughput
  • Online project management system
  • Minimal pricing from cost-recovery core
  • 3-6 week turnaround on most projects
  • Rapid response projects accepted

 

The HiSeq X Ten provides unprecedented capacity, speed and accuracy for sequencing individuals’ entire genetic make-up or DNA — their “whole genome.” HudsonAlpha integrates the HiSeq X Ten into its current high-volume production environment at its Genomic Services Laboratory. The technology makes it possible for HudsonAlpha to produce and analyze genomic data on timescales and at a cost that are relevant to enable routine human whole genome sequencing.

DNA Services

Next-Generation Sequencing:

Whole genome sequencing – all species from human to bacterial genomes

Exome sequencing – reduce the complexity of sequencing the human genome down to the coding regions

Custom capture – isolate and deep-sequence a specific region of the genome

Reduced representation bisulfate sequencing – reduce the amount of sequencing required for methylation analysis

ChIP/amplicon/4C/5C/HiC/other – we can create sequence libraries from outside samples for many protocols with custom specifications

Microarray Services:

Genome-wide SNP mapping –  Options for Illumina Omni Arrays and Affymetrix Axiom

Panels assays – Targeted SNP analysis for follow-up to whole genome analysis

SNP Validation – real-time PCR services with either TaqMan or SYBR Green based detection

Methylation analysis – interrogate over 450K CpG loci covering the entire RefSeq transcriptome

RNA Services

Next-Generation Sequencing:

RNA-Seq – characterize mRNA from total RNA with poly(A) selection or ribosomal RNA (rRNA) reduction

Directional RNA-Seq – characterize mRNA while retaining strand specificity with poly(A) selection or ribosomal RNA (rRNA) reduction

Low-input RNA-Seq – characterize mRNA and non-coding RNA starting with total RNA inputs of less than 10 ng

Small RNA-Seq – characterize microRNA

Microarray:

BeadArray – detect expression levels of known mRNA

De novo genome assembly and finishing

Collaborate with one of the few centers in the world capable of de novo genome sequence and assembly. The Genome Sequencing Center (GSC) has produced high quality plant and fungal genomic resources for over 100 species. We specialize in eukaryotic whole genome sequence, assembly, and analysis together with improvement or finishing of complex eukaryotic genomes and are currently focused on agriculture and bioenergy.

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