BioTech Bootcamp Summer 2010 Tentative
Monday | Tuesday | Wednesday | Thursday | Friday | Monday | |
8:00 AM | Welcome (Classroom) | Recap of Monday & questions | Recap of Tuesday & questions | Recap of Wednesday & questions | Recap of Thursday & questions | Classroom Recap of last week |
Planning & Documenting Laboratory Dealing with complicated protocols | -Practical dilution exercise. -Plating bacteria to get a concentration. | Growing bacterial cells | Protein purification – GPF from pGLO transformed cells | Student Assessment of Biotech Bootcamp | ||
9:00 AM | Registration (auditorium) | Associate Company roundtable discussion | ||||
Centrifugation | ||||||
Measuring, adjusting and controlling pH | G-MOD lab II | |||||
10:00 AM | Tour of laboratory and floor (pix taken while on tour) | Using laboratory balances | Centrifugation exercise | |||
-Measuring pH of a liquid -Proper use of a pH meter -Calibrating a pH meter | ||||||
Reproducibility and Accuracy of Measurements – Data Analysis Using Excel | Bacterial plasmid miniprep | Protein assay – colorimetric | Research Lab roundatable discussions | |||
11:00 AM | Introduction and general orientation | |||||
Buffers – What are they and why are they used? | Spectrophotometry (cont.) | |||||
General laboratory safety | ||||||
12:00 PM | Lunch | Lunch | Lunch | Lunch | Lunch | Lunch |
1:00 PM | Laboratory safety exercise | Non-sterile vs. sterile techniques | Buffers – comparison of titrating a salt solution and a buffer | Transformation | Enzyme catalysis | Interns report to their respective companies |
Preparation of LB broth and LB agar | ||||||
-Reading MSDS sheets -Finding safety information on chemical bottles | ||||||
2:00 PM | Preparation of Glycine/Mg buffer (pH 7.0, pH 8.0, pH 9.0 & pH 10.0) Preparation of PNPP | G-MOD lab I | ||||
Handling and measuring liquids II. Stock solutions and dilutions | ||||||
3:00 PM | Units and the metric system | |||||
-Preparing 1X TAE -Serial two-fold dilutions | Spectrophotometery | Lab Math IV -Representing data -Semi-log graph paper -Determining rates | ||||
Handling & measuring liquids I. Pipets and other measuring devices | How to prepare Powerpoint material: A Powerpoint tutorial for posters and presentations | |||||
4:00 PM | ||||||
Reading a scientific paper | Reading a scientific paper (Part II) | Reading a scientific paper (Part III) | ||||
Rainbow pipeting lab | ||||||
Attached document: